Article
Upf1 stimulates degradation of the product derived from aberrant messenger RNA containing a specific nonsense mutation by the proteasome.
EMBO reports - 1 Nov 2009
Kuroha Kazushige, Tatematsu Tsuyako, Inada Toshifumi
Abstract excerpt
Aberrant messenger RNAs containing a premature termination codon (PTC) are eliminated by the nonsense-mediated mRNA decay (NMD) pathway. Here, we show that a crucial NMD factor, up frameshift 1 protein (Upf1), is required for rapid proteasome-mediated degradation of an aberrant protein (PTC product) derived from a PTC-containing mRNA. Western blot and pulse-chase analyses revealed that Upf1 stimulates the...
Topics
- 3' Untranslated Regions
- Blotting, Western
- Codon, Nonsense
- Codon, Terminator
- Gene Expression Regulation, Fungal
- Models, Genetic
- Mutation
- Proteasome Endopeptidase Complex
- RNA Helicases
- RNA Stability