Article
Minimal promoter systems reveal the importance of conserved residues in the B-finger of human transcription factor IIB.
The Journal of biological chemistry - 11 Sept 2009
Thompson Nancy E, Glaser Bryan T, Foley Katherine M, Burton Zachary F, Burgess Richard R
Abstract excerpt
The "B-finger" of transcription factor IIB (TFIIB) is highly conserved and believed to play a role in the initiation process. We performed alanine substitutions across the B-finger of human TFIIB, made change-of-charge mutations in selected residues, and substituted the B-finger sequence from other organisms. Mutant proteins were examined in two minimal promoter systems (containing only RNA polymerase II,...
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