Article
Assembly of transcription factor IIB at a promoter in vivo requires contact with RNA polymerase II.
EMBO reports - 1 Sept 2006
Elsby Laura M, O'Donnell Amanda J M, Green Laura M, Sharrocks Andrew D, Roberts Stefan G E
Abstract excerpt
The general transcription factor TFIIB has a central role in the assembly of the preinitiation complex at the promoter, providing a platform for the entry of RNA polymerase II/TFIIF. We used an RNA interference (RNAi)-based system in which TFIIB expression is ablated in vivo and replaced with a TFIIB derivative that contains a silent mutation and is refractory to the RNAi. Using this approach, we found that...
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