Article
Low-fidelity DNA synthesis by the L979F mutator derivative of Saccharomyces cerevisiae DNA polymerase zeta.
Nucleic acids research - 1 Jun 2009
Stone Jana E, Kissling Grace E, Lujan Scott A, Rogozin Igor B, Stith Carrie M, Burgers Peter M J, Kunkel Thomas A
Abstract excerpt
To probe Pol zeta functions in vivo via its error signature, here we report the properties of Saccharomyces cerevisiae Pol zeta in which phenyalanine was substituted for the conserved Leu-979 in the catalytic (Rev3) subunit. We show that purified L979F Pol zeta is 30% as active as wild-type Pol zeta when replicating undamaged DNA. L979F Pol zeta shares with wild-type Pol zeta the ability to perform moderately...
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