Article
Inefficient proofreading and biased error rates during inaccurate DNA synthesis by a mutant derivative of Saccharomyces cerevisiae DNA polymerase delta.
The Journal of biological chemistry - 26 Jan 2007
Nick McElhinny Stephanie A, Stith Carrie M, Burgers Peter M J, Kunkel Thomas A
Abstract excerpt
DNA polymerase delta (pol delta) is a high fidelity eukaryotic enzyme that participates in DNA repair and is essential for DNA replication. Toward the goal of dissecting its multiple biological functions, here we describe the biochemical properties of Saccharomyces cerevisiae pol delta with a methionine replacing conserved leucine 612 at the polymerase active site. Compared with wild type pol delta, L612M pol...
Topics
- Amino Acid Sequence
- Catalysis
- DNA Polymerase III
- DNA Replication
- DNA, Fungal
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Protein Conformation
- Saccharomyces cerevisiae