Article
In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta.
Genetics - 1 Sept 2001
Pavlov Y I, Shcherbakova P V, Kunkel T A
Abstract excerpt
Several amino acids in the active site of family A DNA polymerases contribute to accurate DNA synthesis. For two of these residues, family B DNA polymerases have conserved tyrosine residues in regions II and III that are suggested to have similar functions. Here we replaced each tyrosine with alanine in the catalytic subunits of yeast DNA polymerases alpha, delta, epsilon, and zeta and examined the consequences...
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