Article
Critical sequences in the core of the P1 plasmid replication origin.
Journal of bacteriology - 1 Jul 1991
Brendler T, Abeles A, Austin S
Abstract excerpt
The core of the P1 plasmid replication origin consists of a series of 7-bp repeats and a G+C-rich stretch. Methylation of the GATC sequences in the repeats is essential. Forty different single-base mutations in the region were isolated and assayed for origin function. A single-base change within any 7-bp repeat could block the origin, irrespective of whether GATC bases were affected. The repeats themselves were...
Topics
- Bacterial Proteins
- Bacteriophages
- Base Composition
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA, Viral
- DNA-Binding Proteins
- Methylation
- Molecular Sequence Data
- Mutagenesis
- Phenotype