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Article

New non-detrimental DNA-binding mutants of the Escherichia coli initiator protein DnaA.

Journal of molecular biology - 28 Jan 2005

Asklund M, Atlung T

Abstract excerpt

The initiator protein DnaA has several unique DNA-binding features. It binds with high affinity as a monomer to the nonamer DnaA box. In the ATP form, DnaA binds cooperatively to the low-affinity ATP-DnaA boxes, and to single-stranded DNA in the 13mer region of the origin. We have carried out an extensive mutational analysis of the DNA-binding domain of the Escherichia coli DnaA protein using mutagenic PCR. We...

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Identifiers

PMID: 15588821
DOI: 10.1016/j.jmb.2004.10.092

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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