New non-detrimental DNA-binding mutants of the Escherichia coli initiator protein DnaA.

doi:10.1016/j.jmb.2004.10.092

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Article

New non-detrimental DNA-binding mutants of the Escherichia coli initiator protein DnaA.

Journal of molecular biology - 28 Jan 2005

Asklund M, Atlung T

Abstract excerpt

The initiator protein DnaA has several unique DNA-binding features. It binds with high affinity as a monomer to the nonamer DnaA box. In the ATP form, DnaA binds cooperatively to the low-affinity ATP-DnaA boxes, and to single-stranded DNA in the 13mer region of the origin. We have carried out an extensive mutational analysis of the DNA-binding domain of the Escherichia coli DnaA protein using mutagenic PCR. We...

Topics

Alleles
Amino Acid Sequence
Amino Acid Substitution
Bacterial Proteins
Base Sequence
Binding Sites
Biotin
Chromosomes, Bacterial
DNA
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Flavoproteins

Models, Molecular

Molecular Sequence Data

Mutation

Phenotype

Promoter Regions, Genetic

Source

Open in PubMed

Identifiers

PMID: 15588821
DOI: 10.1016/j.jmb.2004.10.092

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