Article
Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein.
Journal of bacteriology - 1 Jan 1992
Arini A, Tuscan M, Churchward G
Abstract excerpt
To investigate the role of binding sites for Rep initiation protein in the replication of pSC101, a series of plasmids was constructed which carried different combinations of mutations in three binding sites within the minimal origin of replication. Mutation of all three sites reduced the affinit...
Topics
- Bacterial Proteins
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Binding
- Replicon
- Trans-Activators
- Transformation, Genetic