Article
Frequency and spectrum of ENU-induced mutation in the Phi X174 transgene in mouse splenic lymphocytes and their significance to spontaneous transgenic rodent mutation frequencies.
Mutagenesis - 1 Sept 2008
Valentine Carrie R, Rainey Heather F, Farrell Jessica M, Shaddock Joseph G, Dobrovolsky Vasily N, Delongchamp Robert R
Abstract excerpt
A perceived disadvantage of transgenic rodent mutation assays is that spontaneous mutant frequencies are high compared to those of endogenous genes and may consequently reduce sensitivity to induced mutation. We have previously argued that unrepaired G:T mismatches from spontaneous deamination of 5-methylcytosine at CpG sites could be converted to apparent in vivo mutations in the bacterial recovery systems...
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