Article
Direct separation of in vivo and in vitro am3 revertants in transgenic mice carrying the phiX174 am3, cs70 vector.
Environmental and molecular mutagenesis - 1 Jan 2001
Malling H V, Delongchamp R R
Abstract excerpt
Target genes in most transgenic systems have higher spontaneous mutation frequencies than do endogenous mammalian genes. Spontaneous mutations in transgenes predominantly arise from three sources: (1) mutations fixed in the animals, (2) mutations arising from replication errors caused by damage to the DNA that may have occurred in vivo or in vitro and then was fixed during amplification of the vector in vitro,...
Topics
- Animals
- Bacteriophage phi X 174
- DNA Adducts
- DNA Mutational Analysis
- Ethylnitrosourea
- Genetic Vectors
- Mice
- Mice, Transgenic
- Mutagens
- Mutation
- Spleen
- Transformation, Genetic
- Transgenes