Article
In vivo mutation analysis using the ΦX174 transgenic mouse and comparisons with other transgenes and endogenous genes.
Mutation research - 1 Dec 2010
Valentine Carrie R, Delongchamp Robert R, Pearce Mason G, Rainey Heather F, Dobrovolsky Vasily N, Malling Heinrich V, Heflich Robert H
Abstract excerpt
The ΦX174 transgenic mouse was first developed as an in vivo Ames test, detecting base pair substitution (bps) at a single bp in a reversion assay. A forward mutational assay was also developed, which is a gain of function assay that also detects bps exclusively. Later work with both assays focused on establishing that a mutation was fixed in vivo using single-burst analysis: determining the number of mutant...
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