Article
Detection of mutations by single-strand conformation polymorphism (SSCP) analysis and SSCP-hybrid methods.
Current protocols in human genetics - 1 May 2001
Warren W, Hovig E, Smith-Sørensen B, Børresen A L, Fujimura F K, Liu Q, Feng J, Sommer S S
Abstract excerpt
Single-strand conformation polymorphism (SSCP) analysis detects mutations based on the fact that single-nucleotide changes in DNA sequences alter the mobility of single-stranded DNA in nondenaturing gels. Four methods for detecting mutations based on SSCP are described here. (1) Traditional SSCP analysis is technically easy and can be used for screening large numbers of samples. SSCP-hybrid methods detect...
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