Article
Gel-based nonradioactive single-strand conformational polymorphism and mutation detection: limitations and solutions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Gupta Vibhuti, Arora Reetakshi, Gochhait Sailesh, Bairwa Narendra K, Bamezai Rameshwar N K
Abstract excerpt
Single-strand conformation polymorphism (SSCP) for screening mutations/single-nucleotide polymorphisms (SNPs) is a simple, cost-effective technique, saving an expensive exercise of sequencing each and every polymerase chain reaction product and assisting in choosing only the amplicons of interest with expected mutations. The principle of detection of small changes in DNA sequences is based on changes in...
Topics
- Base Sequence
- DNA
- DNA Mutational Analysis
- Electrophoresis, Polyacrylamide Gel
- Humans
- Hydrogen-Ion Concentration
- Polymorphism, Single Nucleotide
- Polymorphism, Single-Stranded Conformational
- Silver Staining