Clonal polymerase chain reaction-single-strand conformational polymorphism analysis: an effective approach for identifying cloned sequences.

doi:10.1016/j.ab.2008.04.005

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Article

Clonal polymerase chain reaction-single-strand conformational polymorphism analysis: an effective approach for identifying cloned sequences.

Analytical biochemistry - 1 Jul 2008

Zhou H, Hickford J G H

Abstract excerpt

The amplification of target sequences from genomic DNA can result in more than one amplicon sequence being produced even when highly specific primers are used. Here we present a clonal polymerase chain reaction-single-strand conformational polymorphism (PCR-SSCP) approach for screening cloned amplicons and identifying particular clones prior to sequence determination. Comparison of the PCR-SSCP patterns of the...

Topics

Alleles
Animals
Base Sequence
Cloning, Molecular
Genome
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Sequence Alignment
Sheep

Source

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Identifiers

PMID: 18435904
DOI: 10.1016/j.ab.2008.04.005

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