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Article

Detection of mutations and polymorphisms using fluorescence-based dideoxy fingerprinting (F-ddF).

BioTechniques - 1 Oct 1994

Ellison J, Squires G, Crutchfield C, Goldman D

Abstract excerpt

We have adapted the dideoxy finger-printing (ddF) technique for detecting DNA sequence variants to fluorescence detection (F-ddF) using an Applied Biosystems Model 373A DNA Sequencer equipped with GENESCAN 672 software and an external temperature control device. The fingerprints can be precisely aligned using an internal standard run in the same lanes. This facilitates location and characterization of mobility...

Topics

Animals
Base Sequence
DNA Fingerprinting
Fluorescence
Globins
Introns
Mice
Molecular Sequence Data
Mutation
Polymorphism, Genetic
Polymorphism, Single-Stranded Conformational

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

Sequence Analysis, DNA

Temperature

Source

Identifiers

PMID: 7833039

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7833039-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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