Article
A comparison of fluorescent SSCP and denaturing HPLC for high throughput mutation scanning.
Human mutation - 1 Jan 2000
Ellis L A, Taylor C F, Taylor G R
Abstract excerpt
We examined 67 different mutations in 16 different amplicons in a comparison of mutation detection by fluorescent single strand conformation polymorphism (F-SSCP) and by denaturing HPLC (DHPLC). F-SSCP was used to analyze fluorescent amplicons with internal size standards and automated fragment analysis (GeneScan, PE Applied Biosystems, Foster City, CA). In DHPLC, unlabelled amplicons were analyzed by reverse...
Topics
- Chromatography, High Pressure Liquid
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA Mutational Analysis
- Exons
- Genotype
- Humans
- Ligases
- Mutation
- Polymorphism, Single-Stranded Conformational
- Proteins
- Reproducibility of Results