Article
Gel-based nonradioactive single-strand conformational polymorphism and mutation detection: limitations and solutions.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005
Gupta Vibhuti, Arora Reetakshi, Ranjan Anand, Bairwa Narendra K, Malhotra Dheeraj K, Udhayasuriyan P T, Saha Anjana, Bamezai Ramesh
Abstract excerpt
Single-strand conformation polymorphism (SSCP) for screening mutations/single-nucleotide polymorphisms (SNPs) is a simple, cost-effective technique, saving an expensive exercise of sequencing each and every PCR reaction product and assisting in choosing only the amplicons of interest with expected mutation. The principle of detection of small changes in DNA sequences is based on the changes in single-strand DNA...
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