Fluorescence-based mutation detection. Single-strand conformation polymorphism analysis (F-SSCP).

doi:10.1007/BF02762409

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Article

Fluorescence-based mutation detection. Single-strand conformation polymorphism analysis (F-SSCP).

Molecular biotechnology - 1 Feb 1996

Ellison J S

Abstract excerpt

Conventional SSCP analysis of DNA amplified by polymerase chain reaction (PCR-SSCP) is one of the simplest and most reliable tools for identifying point mutations, and small insertions or deletions. The sensitivity of the technique is increased by using the Applied Biosystems (ABI) semiautomated...

Topics

DNA Primers
Electrophoresis, Polyacrylamide Gel
Fluorescence
Methods
Mutation
Polymorphism, Single-Stranded Conformational
Sequence Analysis, DNA

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