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Article

Directed evolution of yeast pyruvate decarboxylase 1 for attenuated regulation and increased stability.

Biochemistry - 4 Mar 2008

Stevenson Bradley J, Liu Jian-Wei, Ollis David L

Abstract excerpt

Five generations of directed evolution resulted in yeast pyruvate decarboxylase 1 (Pdc1) variants with improved activity for 1 mM pyruvate at pH 7.5 in the presence of phosphate. The best variant, named 5LS30, contained the following mutations: A143T, T156A, Q367H, N396I, and K478R. In comparison with native Pdc1, 5LS30 had the substrate concentration required for half-saturation reduced by almost 3-fold at pH...

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PMID: 18232643
DOI: 10.1021/bi701858u

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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