Article
Engineering of yeast pyruvate decarboxylase for enhanced selectivity towards carboligation.
Bioresource technology - 1 Sept 2015
Agarwal Praveen Kumar, Uppada Vanita, Swaminathan A G, Noronha Santosh B
Abstract excerpt
The aim of the study was to increase production of (R)-PAC by altering carboligation activity of Pdc in Saccharomyces cerevisiae. Pdc1 activity was modified by over-expression as well as changing the rate of decarboxylation and carboligation by site specific mutation in Pdc1. Over-expression of mutant Pdc1 resulted in 50 ± 2.5% increase in levels of (R)-PAC in wild-type and further 30-40% in pdc null background....
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