Article
Directed evolution of pyruvate decarboxylase-negative Saccharomyces cerevisiae, yielding a C2-independent, glucose-tolerant, and pyruvate-hyperproducing yeast.
Applied and environmental microbiology - 1 Jan 2004
van Maris Antonius J A, Geertman Jan-Maarten A, Vermeulen Alexander, Groothuizen Matthijs K, Winkler Aaron A, Piper Matthew D W, van Dijken Johannes P, Pronk Jack T
Abstract excerpt
The absence of alcoholic fermentation makes pyruvate decarboxylase-negative (Pdc(-)) strains of Saccharomyces cerevisiae an interesting platform for further metabolic engineering of central metabolism. However, Pdc(-) S. cerevisiae strains have two growth defects: (i) growth on synthetic medium in glucose-limited chemostat cultures requires the addition of small amounts of ethanol or acetate and (ii) even in the...
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