Article
pdc1(0) mutants of Saccharomyces cerevisiae give evidence for an additional structural PDC gene: cloning of PDC5, a gene homologous to PDC1.
Journal of bacteriology - 1 Feb 1990
Seeboth P G, Bohnsack K, Hollenberg C P
Abstract excerpt
The PDC1 gene coding for a pyruvate decarboxylase (PDC; EC 4.1.1.1) was deleted from the Saccharomyces cerevisiae genome. The resulting pdc1(0) mutants were able to grow on glucose and still contained 60 to 70% of the wild-type PDC activity. Two DNA fragments with sequences homologous to that of...
Topics
- Blotting, Northern
- Blotting, Southern
- Carboxy-Lyases
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Gene Expression
- Genes, Fungal
- Kinetics
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Pyruvate Decarboxylase
- RNA, Fungal
- Recombinant Proteins
- Restriction Mapping
- Saccharomyces cerevisiae
- Sequence Homology, Nucleic Acid