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Article

A bacterial selection for the directed evolution of pyruvate aldolases.

Bioorganic & medicinal chemistry - 1 Aug 2004

Griffiths Jennifer S, Cheriyan Manoj, Corbell Jayme B, Pocivavsek Luka, Fierke Carol A, Toone Eric J

Abstract excerpt

A novel bacterial in vivo selection for pyruvate aldolase activity is described. Pyruvate kinase deficient cells, which lack the ability to biosynthetically generate pyruvate, require supplementation of exogenous pyruvate when grown on ribose. Supplementation with pyruvate concentrations as low as 50 microM rescues cell growth. A known substrate of the KDPG aldolases, 2-keto-4-hydroxy-4-(2'-pyridyl)butyrate...

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Identifiers

PMID: 15246084
DOI: 10.1016/j.bmc.2004.05.034

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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