Article
DNA binding and dimerization determinants for thyroid hormone receptor alpha and its interaction with a nuclear protein.
Molecular endocrinology (Baltimore, Md.) - 1 Dec 1991
Zhang X K, Tran P B, Pfahl M
Abstract excerpt
The gel retardation assay was used to analyze the role of the thyroid hormone receptor alpha (TR alpha) ligand-binding domain (LBD) in controlling receptor interaction with a thyroid hormone responsive element (TRE). While wild type receptor TR alpha binds to the TRE mainly as monomer, deletion of 85 amino acids from its C-terminus results in a mutant receptor with enhanced DNA binding that forms several slow...
Topics
- Amino Acids
- Animals
- Base Sequence
- DNA
- DNA-Binding Proteins
- HeLa Cells
- Humans
- Leucine Zippers
- Ligands
- Molecular Sequence Data
- Mutation
- Nuclear Proteins