Article
Break-induced loss of heterozygosity in fission yeast: dual roles for homologous recombination in promoting translocations and preventing de novo telomere addition.
Molecular and cellular biology - 1 Nov 2007
Cullen Jason K, Hussey Sharon P, Walker Carol, Prudden John, Wee Boon-Yu, Davé Anoushka, Findlay James S, Savory Andrew P, Humphrey Timothy C
Abstract excerpt
Loss of heterozygosity (LOH), a causal event in tumorigenesis, frequently encompasses multiple genetic loci and whole chromosome arms. However, the mechanisms leading to such extensive LOH are poorly understood. We investigated the mechanisms of DNA double-strand break (DSB)-induced extensive LOH by screening for auxotrophic marker loss approximately 25 kb distal to an HO endonuclease break site within a...
Topics
- Alleles
- Base Sequence
- Chromosomes, Fungal
- Crossing Over, Genetic
- DNA Breaks, Double-Stranded
- DNA Repair
- Genetic Markers
- Loss of Heterozygosity
- Models, Genetic
- Molecular Sequence Data
- Multiprotein Complexes
- Mutation