Article
Phorbol diester-induced alterations in the expression of protein kinase C isozymes and their mRNAs. Analysis in wild-type and phorbol diester-resistant HL-60 cell clones.
The Journal of biological chemistry - 15 Aug 1991
McSwine-Kennick R L, McKeegan E M, Johnson M D, Morin M J
Abstract excerpt
In an HL-60 cell subline (PR-17) which was greater than 100-fold resistant to the differentiating and cytostatic activities of phorbol 12-myristate 13-acetate (PMA), the protein kinase C phenotype was found to be nearly identical to that of wild-type HL-60 cells. A measurable decrease (30%) in th...
Topics
- Animals
- Blotting, Northern
- Blotting, Western
- Catalysis
- Chromatography, Liquid
- Gene Expression Regulation, Enzymologic
- Isoenzymes
- Kinetics
- Molecular Sequence Data
- Phenotype
- Poly A
- Protein Kinase C
- RNA
- RNA, Messenger
- Rats
- Tetradecanoylphorbol Acetate
- Transcription, Genetic
- Tumor Cells, Cultured