Article
Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes.
RNA (New York, N.Y.) - 1 Jan 2007
Kim Kevin, Lee Young Sik, Carthew Richard W
Abstract excerpt
In the Drosophila RNA interference (RNAi) pathway, small interfering RNAs (siRNAs) direct Argonaute2 (Ago2), an endonuclease, within the RNA-induced silencing complex (RISC) to cleave complementary mRNA targets. In vitro studies have shown that, for each siRNA duplex, RISC retains only one strand, the guide, and releases the other, the passenger, to form a holo-RISC complex. Here, we have isolated a new Ago2...
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