Article
C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.
Science (New York, N.Y.) - 7 Aug 2009
Liu Ying, Ye Xuecheng, Jiang Feng, Liang Chunyang, Chen Dongmei, Peng Junmin, Kinch Lisa N, Grishin Nick V, Liu Qinghua
Abstract excerpt
The catalytic engine of RNA interference (RNAi) is the RNA-induced silencing complex (RISC), wherein the endoribonuclease Argonaute and single-stranded small interfering RNA (siRNA) direct target mRNA cleavage. We reconstituted long double-stranded RNA- and duplex siRNA-initiated RISC activities with the use of recombinant Drosophila Dicer-2, R2D2, and Ago2 proteins. We used this core reconstitution system to...
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