Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Native gel analysis for RISC assembly.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011

Kawamata Tomoko, Tomari Yukihide

Abstract excerpt

Small-interfering RNAs (siRNAs) and microRNAs (miRNAs) regulate expression of their target mRNAs via the RNA-induced silencing complex (RISC). A core component of RISC is the Argonaute (Ago) protein, which dictates the RISC function. In Drosophila, miRNAs and siRNAs are generally loaded into Ago1-containing RISC (Ago1-RISC) and Ago2-containing RISC (Ago2-RISC), respectively. We developed a native agarose gel...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Argonaute Proteins
Share to Electrophoresis, Agar Gel
Share to Eukaryotic Initiation Factors

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Isotope Labeling

Share to Ribonuclease III

Share to RNA Helicases

Share to RNA-Induced Silencing Complex

Source

Identifiers

PMID: 21528449
DOI: 10.1007/978-1-61779-046-1_7

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Native gel analysis for RISC assembly.PMID 21528449Read ↗

Conversion of pre-RISC to holo-RISC by Ago2 during assembly of RNAi complexes.Semantic proximity Read ↗

Passenger-strand cleavage facilitates assembly of siRNA into Ago2-containing RNAi enzyme complexes.Semantic proximity Read ↗

C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation.Semantic proximity Read ↗

The RNAi pathway initiated by Dicer-2 in Drosophila.Semantic proximity Read ↗

Identification of specific let-7 microRNA binding complexes in Caenorhabditis elegans.Semantic proximity Read ↗

The Integrator complex regulates microRNA abundance through RISC loadingSemantic proximity Read ↗

A potential protein-RNA recognition event along the RISC-loading pathway from the structure of A. aeolicus Argonaute with externally bound siRNA.Semantic proximity Read ↗

VCP Machinery Mediates Autophagic Degradation of Empty ArgonauteSemantic proximity Read ↗

Argonaute proteins regulate microRNA stability: Increased microRNA abundance by Argonaute proteins is due to microRNA stabilization.Semantic proximity Read ↗

RISC assembly defects in the Drosophila RNAi mutant armitage.Semantic proximity Read ↗

R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway.Semantic proximity Read ↗

Agarose overlays allow simplified staining of polyacrylamide gels.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.