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Article

Detection of BRAF V600E mutation in colorectal cancer: comparison of automatic sequencing and real-time chemistry methodology.

The Journal of molecular diagnostics : JMD - 1 Nov 2006

Benlloch Susana, Payá Artemio, Alenda Cristina, Bessa Xavier, Andreu Montserrat, Jover Rodrigo, Castells Antoni, Llor Xavier, Aranda F Ignacio, Massutí Bartomeu

Abstract excerpt

Mutation V600E of BRAF, a kinase-encoding gene from the RAS/RAF/MAPK pathway, in colorectal carcinoma (CRC) suggests a sporadic origin of the disease, providing an exclusion criterion for hereditary nonpolyposis colorectal cancer. Here we describe detection of this mutation by real-time chemistry TaqMan MGB probes, confirmed by direct DNA sequencing as the gold standard. DNA was extracted from paraffin-embedded...

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Share to Colorectal Neoplasms
Share to Colorectal Neoplasms, Hereditary Nonpolyposis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Mutational Analysis

Share to MSH2 gene

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Share to Proto-Oncogene Proteins B-raf

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Source

Open in PubMed Open full text

Identifiers

PMID: 17065421
DOI: 10.2353/jmoldx.2006.060070
PMCID: PMC1876165

Genes

BRAF
MLH1
MSH2

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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