Article
Rapid detection of mutations in the BRAF gene using real-time polymerase chain reaction and melting curve analysis.
Cancer genetics and cytogenetics - 1 Feb 2004
Ikenoue Tsuneo, Hikiba Yohko, Kanai Fumihiko, Ijichi Hideaki, Togo Goichi, Ohta Miki, Watabe Hirotsugu, Yamaji Yutaka, Okamoto Makoto, Aragaki Jun, Matsumura Masayuki, Kawabe Takao, Omata Masao
Abstract excerpt
The BRAF gene is mutated in 66% of melanomas and less frequently in various human cancers. More than 80% of these mutations are T to A transversions at nucleotide 1796 (T1796A), leading to a substitution of glutamic acid for valine at amino acid 599 (V599E). We established a new method for rapidly detecting V599E mutations using real-time polymerase chain reaction and melting curve analysis. Furthermore, we...
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