Article
The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer.
The Journal of molecular diagnostics : JMD - 1 Sept 2010
Mancini Irene, Santucci Claudio, Sestini Roberta, Simi Lisa, Pratesi Nicola, Cianchi Fabio, Valanzano Rosa, Pinzani Pamela, Orlando Claudio
Abstract excerpt
Fast and reliable tests to detect mutations in human cancers are required to better define clinical samples and orient targeted therapies. KRAS mutations occur in 30-50% of colorectal cancers (CRCs) and represent a marker of clinical resistance to cetuximab therapy. In addition, the BRAF V600E is mutated in about 10% of CRCs, and the development of a specific inhibitor of mutant BRAF kinase has prompted a growing...
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