Article
Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets.
Journal of molecular biology - 20 Jan 2006
Arnould Sylvain, Chames Patrick, Perez Christophe, Lacroix Emmanuel, Duclert Aymeric, Epinat Jean-Charles, Stricher François, Petit Anne-Sophie, Patin Amélie, Guillier Sophie, Rolland Sandra, Prieto Jesús, Blanco Francisco J, Bravo Jerónimo, Montoya Guillermo, Serrano Luis, Duchateau Philippe, Pâques Frédéric
Abstract excerpt
The last decade has seen the emergence of a universal method for precise and efficient genome engineering. This method relies on the use of sequence-specific endonucleases such as homing endonucleases. The structures of several of these proteins are known, allowing for site-directed mutagenesis of residues essential for DNA binding. Here, we show that a semi-rational approach can be used to derive hundreds of...
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