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Article

An in vivo selection system for homing endonuclease activity.

Nucleic acids research - 1 Apr 2002

Gruen Mathias, Chang Kathy, Serbanescu Irina, Liu David R

Abstract excerpt

Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro...

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Identifiers

PMID: 11917035
DOI: 10.1093/nar/30.7.e29
PMCID: PMC101853

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An in vivo selection system for homing endonuclease activity.PMID 11917035Read ↗

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