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Article

Context dependence between subdomains in the DNA binding interface of the I-CreI homing endonuclease.

Nucleic acids research - 1 Aug 2011

Grizot Sylvestre, Duclert Aymeric, Thomas Séverine, Duchateau Philippe, Pâques Frédéric

Abstract excerpt

Homing endonucleases (HE) have emerged as precise tools for achieving gene targeting events. Redesigned HEs with tailored specificities can be used to cleave new sequences, thereby considerably expanding the number of targetable genes and loci. With HEs, as well as with other protein scaffolds, context dependence of DNA/protein interaction patterns remains one of the major limitations for rational engineering of...

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Identifiers

PMID: 21482539
DOI: 10.1093/nar/gkr186
PMCID: PMC3152339

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