Article
Mutations altering the cleavage specificity of a homing endonuclease.
Nucleic acids research - 1 Sept 2002
Seligman Lenny M, Chisholm Karen M, Chevalier Brett S, Chadsey Meggen S, Edwards Samuel T, Savage Jeremiah H, Veillet Adeline L
Abstract excerpt
The homing endonuclease I-CreI recognizes and cleaves a particular 22 bp DNA sequence. The crystal structure of I-CreI bound to homing site DNA has previously been determined, leading to a number of predictions about specific protein-DNA contacts. We test these predictions by analyzing a set of endonuclease mutants and a complementary set of homing site mutants. We find evidence that all structurally predicted...
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