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Article

Creation of a type IIS restriction endonuclease with a long recognition sequence.

Nucleic acids research - 1 May 2009

Lippow Shaun M, Aha Patti M, Parker Matthew H, Blake William J, Baynes Brian M, Lipovsek Dasa

Abstract excerpt

Type IIS restriction endonucleases cleave DNA outside their recognition sequences, and are therefore particularly useful in the assembly of DNA from smaller fragments. A limitation of type IIS restriction endonucleases in assembly of long DNA sequences is the relative abundance of their target sites. To facilitate ligation-based assembly of extremely long pieces of DNA, we have engineered a new type IIS...

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Identifiers

PMID: 19304757
DOI: 10.1093/nar/gkp182
PMCID: PMC2685105

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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