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Article

Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds.

Nucleic acids research - 1 Apr 2010

Grizot Sylvestre, Epinat Jean-Charles, Thomas Séverine, Duclert Aymeric, Rolland Sandra, Pâques Frédéric, Duchateau Philippe

Abstract excerpt

Homing endonucleases have become valuable tools for genome engineering. Their sequence recognition repertoires can be expanded by modifying their specificities or by creating chimeric proteins through domain swapping between two subdomains of different homing endonucleases. Here, we show that these two approaches can be combined to create engineered meganucleases with new specificities. We demonstrate the...

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Identifiers

PMID: 20026587
DOI: 10.1093/nar/gkp1171
PMCID: PMC2847234

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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