Article
Generation of a nicking enzyme that stimulates site-specific gene conversion from the I-AniI LAGLIDADG homing endonuclease.
Proceedings of the National Academy of Sciences of the United States of America - 31 Mar 2009
McConnell Smith Audrey, Takeuchi Ryo, Pellenz Stefan, Davis Luther, Maizels Nancy, Monnat Raymond J, Stoddard Barry L
Abstract excerpt
Homing endonucleases stimulate gene conversion by generating site-specific DNA double-strand breaks that are repaired by homologous recombination. These enzymes are potentially valuable tools for targeted gene correction and genome engineering. We have engineered a variant of the I-AniI homing endonuclease that nicks its cognate target site. This variant contains a mutation of a basic residue essential for proton...
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