Article
An essential tryptophan of Escherichia coli DnaA protein functions in oligomerization at the E. coli replication origin.
The Journal of biological chemistry - 1 Jul 2005
Felczak Magdalena M, Simmons Lyle A, Kaguni Jon M
Abstract excerpt
In the initiation of bacterial DNA replication, DnaA protein recruits DnaB helicase to the chromosomal origin, oriC, leading to the assemble of the replication fork machinery at this site. Because a region near the N terminus of DnaA is required for self-oligomerization and the loading of DnaB helicase at oriC, we asked if these functions are separable or interdependent by substituting many conserved amino acids...
Topics
- Adenosine Triphosphate
- Alanine
- Amino Acid Motifs
- Bacterial Proteins
- Cross-Linking Reagents
- DNA
- DNA Mutational Analysis
- DNA-Binding Proteins
- Dose-Response Relationship, Drug
- Escherichia coli
- Glutaral
- Leucine
- Mutation
- Phenylalanine