Article
Threonine 435 of Escherichia coli DnaA protein confers sequence-specific DNA binding activity.
The Journal of biological chemistry - 12 Sept 1997
Sutton M D, Kaguni J M
Abstract excerpt
The Escherichia coli DnaA protein, as a sequence-specific DNA binding protein, promotes the initiation of chromosomal replication by binding to four asymmetric 9-mer sequences termed DnaA boxes in oriC. Characterization of N-terminal, C-terminal, and internal in-frame deletion mutants identified...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Bacterial Proteins
- Binding, Competitive
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Structure, Secondary
- Recombinant Proteins
- Sequence Deletion
- Structure-Activity Relationship
- Threonine