Article
The invertor knock-in conditional chromosomal translocation mimic.
Nature methods - 1 Jan 2005
Forster Alan, Pannell Richard, Drynan Lesley F, Codrington Rosalind, Daser Angelika, Metzler Markus, Lobato M Natividad, Rabbitts Terence H
Abstract excerpt
Knock-in models of tumor-specific chromosomal translocations can generate lethal mutations. To circumvent this, a new conditional gene fusion model has been developed (invertor mice) and exemplified with the Ews-ERG fusion oncogene. An ERG segment, flanked by loxP sites, was knocked in to an intron of the Ews gene but in an inverted transcription orientation and lineage-specific Ews-ERG fusion created by...
Topics
- Alleles
- Animals
- Base Sequence
- Cell Separation
- Chromosomes
- DNA, Complementary
- Flow Cytometry
- Genetic Techniques
- Humans
- Introns
- Mice
- Mice, Inbred C57BL
- Models, Genetic
- Molecular Sequence Data
- Mutation