Article
Improved methods for the generation of human gene knockout and knockin cell lines.
Nucleic acids research - 7 Oct 2005
Topaloglu Ozlem, Hurley Paula J, Yildirim Ozlem, Civin Curt I, Bunz Fred
Abstract excerpt
Recent studies have demonstrated the utility of recombinant adeno-associated viral (rAAV) vectors in the generation of human knockout cell lines. The efficiency with which such cell lines can be generated using rAAV, in comparison with more extensively described plasmid-based approaches, has not been directly tested. In this report, we demonstrate that targeting constructs delivered by rAAV vectors were nearly...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.