Article
Generation of mouse conditional knockout alleles in one step using the i-GONAD method.
Genome research - 1 Jan 2021
Shang Renjie, Zhang Haifeng, Bi Pengpeng
Abstract excerpt
The Cre/loxP system is a powerful tool for gene function study in vivo. Regulated expression of Cre recombinase mediates precise deletion of genetic elements in a spatially- and temporally-controlled manner. Despite the robustness of this system, it requires a great amount of effort to create a conditional knockout model for each individual gene of interest where two loxP sites must be simultaneously inserted in...
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