Article
DNA polymerase V and RecA protein, a minimal mutasome.
Molecular cell - 18 Feb 2005
Schlacher Katharina, Leslie Kris, Wyman Claire, Woodgate Roger, Cox Michael M, Goodman Myron F
Abstract excerpt
A hallmark of the Escherichia coli SOS response is the large increase in mutations caused by translesion synthesis (TLS). TLS requires DNA polymerase V (UmuD'2C) and RecA. Here, we show that pol V and RecA interact by two distinct mechanisms. First, pol V binds to RecA in the absence of DNA and ATP and second, through its UmuD' subunit, requiring DNA and ATP without ATP hydrolysis. TLS occurs in the absence of a...
Topics
- Adenosine Triphosphate
- DNA Damage
- DNA Replication
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Mutagenesis
- Mutation
- Rec A Recombinases