Article
Conformational regulation of Escherichia coli DNA polymerase V by RecA and ATP.
PLoS genetics - 1 Feb 2019
Jaszczur Malgorzata M, Vo Dan D, Stanciauskas Ramunas, Bertram Jeffrey G, Sikand Adhirath, Cox Michael M, Woodgate Roger, Mak Chi H, Pinaud Fabien, Goodman Myron F
Abstract excerpt
Mutagenic translesion DNA polymerase V (UmuD'2C) is induced as part of the DNA damage-induced SOS response in Escherichia coli, and is subjected to multiple levels of regulation. The UmuC subunit is sequestered on the cell membrane (spatial regulation) and enters the cytosol after forming a UmuD'2C complex, ~ 45 min post-SOS induction (temporal regulation). However, DNA binding and synthesis cannot occur until...
Read the complete abstract on PubMedTopics
- Adenosine Triphosphate
- DNA Damage
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Enzyme Activation
- Escherichia coli