Article
RecA acts in trans to allow replication of damaged DNA by DNA polymerase V.
Nature - 24 Aug 2006
Schlacher Katharina, Cox Michael M, Woodgate Roger, Goodman Myron F
Abstract excerpt
The DNA polymerase V (pol V) and RecA proteins are essential components of a mutagenic translesion synthesis pathway in Escherichia coli designed to cope with DNA damage. Previously, it has been assumed that RecA binds to the DNA template strand being copied. Here we show, however, that pol-V-catalysed translesion synthesis, in the presence or absence of the beta-processivity-clamp, occurs only when RecA...
Topics
- Catalysis
- DNA Damage
- DNA Replication
- DNA, Single-Stranded
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Mutation
- Rec A Recombinases
- SOS Response, Genetics