Isolation and characterization of mutant bacteriophage T7 RNA polymerases.

doi:10.1016/0022-2836(92)90996-w

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Article

Isolation and characterization of mutant bacteriophage T7 RNA polymerases.

Journal of molecular biology - 20 Mar 1992

Patra D, Lafer E M, Sousa R

Abstract excerpt

We have isolated and characterized a number of bacteriophage T7 RNAP (RNA polymerase) null mutants. Most of the mutants found to be completely inactive in vitro map to one of the well-conserved blocks of residues in the family of RNAPs homologous to T7 RNAP. The in vitro phenotypes of a smaller n...

Topics

Catalysis
DNA-Directed RNA Polymerases
Kinetics
Mutagenesis
Mutation
Promoter Regions, Genetic
Restriction Mapping
Structure-Activity Relationship
T-Phages
Transcription, Genetic

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