Article
[Genetic system of selecting point mutations of bacteriophage T7 RNA polymerase].
Molekuliarnaia biologiia - 1 Jan 2000
Rechinskiĭ V O, Dragan S M, Kostiuk D A, Liakhov D L, Kochetkov S N
Abstract excerpt
Random mutagenesis of the bacteriophage T7 gene 1 was used to delineate the functionally important amino acids residues of its product--T7 RNA polymerase. A two-plasmid system has been constructed for the phenotypic selection of the mutants. Nine mutants were isolated; the RNA polymerase with Tyr...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- DNA-Directed RNA Polymerases
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Phenotype
- Plasmids
- Sequence Homology, Nucleic Acid
- T-Phages